![]() ![]() Inappropriate initiation of replication at late origins, because of loss of the checkpoint, probably causes the elevated level of chromatin-bound replisome proteins in the ctf18Δ mutant. We found that ctf18Δ cells are also defective in Rad53 activation, revealing that the Ctf18 complex is required for engagement of the DNA replication checkpoint. Chromatin composition abnormalities observed in ctf18Δ cells were very similar to those of an mrc1Δ mutant, which is defective in the activating the Rad53 checkpoint kinase in response to DNA replication stress. Compared with wild type, hydroxyurea-treated ctf18Δ cells exhibited increased chromatin association of replisome progression complex components including Cdc45, Ctf4, and GINS complex subunits, the polymerase processivity clamp PCNA and the single-stranded DNA-binding complex RPA. ![]() The most marked abnormalities occurred when cells were challenged with the replication inhibitor hydroxyurea. Three hundred and seven of the 491 reported chromosomal proteins were quantitated. To understand the in vivo function of the Ctf18 complex, we analyzed chromatin composition in a ctf18Δ mutant using the quantitative proteomic technique of stable isotope labeling by amino acids in cell culture. 15 ******************************************************************************/ 16 package ġ7 18 import java.io.BufferedInputStream Ģ9 import ĥ3 54 import ĥ5 import ĥ7 import ĥ8 import Ħ6 67 import. Ħ8 import .GrouperInstallerIndexFile.PatchFileType Ħ9 import .util.GiDbUtils ħ0 import .util.GrouperInstallerUtils ħ1 import. ħ2 import. ħ3 import. ħ4 import. ħ5 import .utils.IOUtils ħ6 import .HttpClient ħ7 import .methods.GetMethod ħ8 79 /** 80 * Install grouper 81 * mchyzer 82 * 83 */ 84 public class GrouperInstaller else if (GrouperInstallerUtils.propertiesValueBoolean( " cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability. 13 * See the License for the specific language governing permissions and 14 * limitations under the License. 6 * You may obtain a copy of the License at 7 * 8 * 9 * 10 * Unless required by applicable law or agreed to in writing, software 11 * distributed under the License is distributed on an "AS IS" BASIS, 12 * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. ![]()
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